BEHAVIOR OF ACYL CARRIER PROTEINS ON WESTERN BLOTS

被引：5

作者：

WORSHAM, LMS ^{[1
]}

TUCKER, M ^{[1
]}

ERNSTFONBERG, ML ^{[1
]}

机构：

[1] E TENNESSEE STATE UNIV,QUILLEN DISHNER COLL MED,DEPT BIOCHEM,JOHNSON CITY,TN 37614

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1043卷 / 02期

基金：

美国国家科学基金会;

关键词：

Acyl carrier protein; Western blot;

D O I：

10.1016/0005-2760(90)90296-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acyl carrier proteins (ACPs) from Escherichia coli and Euglena were analyzed on Western blots using rabbit antibodies raised against E. coli ACP. Euglena ACP, unlike that from E. coli, behaves upon electrophoresis under denaturing conditions as its size would predict. Oligomeric forms of both ACPs were evident on Western blots, but the bacterial ACP had more tendency to aggregate. That the oligomeric forms were not due to impurities was shown by their regeneration from low-Mr protein, reaction with antibodies isolated from Iow-Mr protein, and by molecular weight determination of the ACP by low-angle laser light scattering. © 1990.

引用

页码：198 / 202

页数：5

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