ESTROGEN-RECEPTOR LEVEL DETERMINES SEX-SPECIFIC INVITRO TRANSCRIPTION FROM THE XENOPUS VITELLOGENIN PROMOTER

被引：36

作者：

CORTHESY, B ^{[1
]}

CLARET, FX ^{[1
]}

WAHLI, W ^{[1
]}

机构：

[1] UNIV LAUSANNE, INST BIOL ANIM, CH-1015 LAUSANNE, SWITZERLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 20期

关键词：

Hepatocytes; Liver-specific gene expression; Vaccinia virus;

D O I：

10.1073/pnas.87.20.7878

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Female-specific expression of the Xenopus laevis vitellogenin gene was reconstituted in vitro by addition of recombinant vaccinia-virus-produced estrogen receptor to nuclear extracts from male livers, in which this gene is silent. Transcription enhancement was at least 30 times and was selectively restricted to vitellogenin templates containing the estrogen-responsive unit. Thus, in male hepatocytes, estrogen receptor is the limiting regulatory factor that in the female liver controls efficient and accurate sex-specific expression of the vitellogenin gene. Furthermore, the Xenopus liver factor B, which is essential in addition to the estrogen receptor for the activation of this gene, was successfully replaced in the Xenopus extract by purified human nuclear factor I, identifying factor B of Xenopus as a functional homolog of this well-characterized human transcription factor.

引用

页码：7878 / 7882

页数：5

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