BOUND AND UNBOUND PYRIDINE DINUCLEOTIDES IN NORMAL AND GLUCOSE-6-PHOSPHATE DEHYDROGENASE-DEFICIENT ERYTHROCYTES

被引：17

作者：

CANEPA, L ^{[1
]}

FERRARIS, AM ^{[1
]}

MIGLINO, M ^{[1
]}

GAETANI, GF ^{[1
]}

机构：

[1] UNIV GENOA, IST SCI MED INTERNA, DIV HEMATOL, VIALE BENEDETTO XV, I-16132 GENOA, ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1074卷 / 01期

关键词：

NAD(H); NADP(H); GLUCOSE-6-PHOSPHATE DEHYDROGENASE; (HUMAN ERYTHROCYTE);

D O I：

10.1016/0304-4165(91)90046-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have measured, by a sensitive cycling assay, the concentration of bound and unbound dinucleotides in normal and glucose-6-phosphate dehydrogenase (G6PD)-deficient erythrocytes. Measurement of free NADP in ultrafiltrates confirms that in normal erythrocytes almost all NADP is bound to cytosolic proteins. In glucose-6-phosphate dehydrogenase-deficient erythrocytes unbound NADP is significantly higher than in normal red cells and the NADP+/NADPH ratio is largely in favor of the oxidized form. In normal and glucose-6-phosphate dehydrogenase-deficient erythrocytes essentially all NAD (bound and unbound) is in the oxidized state. About 50% of the total amount of NAD (NAD+ + NADH) is free in the cytosol, with a NAD+/NADH ratio greater than 100.

引用

页码：101 / 104

页数：4

共 16 条

[1]

BURCH HB, 1967, J BIOL CHEM, V242, P4546

[2] GENERATION OF HYDROGEN PEROXIDE IN ERYTHROCYTES BY HEMOLYTIC AGENTS [J].

COHEN, G ;

HOCHSTEIN, P .

BIOCHEMISTRY, 1964, 3 (07) :895-&

[3]

GAETANI GF, 1989, BLOOD, V73, P334

[4] EFFECT OF HEMOLYSIS ON HEXOSE MONOPHOSPHATE PATHWAY IN NORMAL AND IN GLUCOSE-6-PHOSPHATE DEHYDROGENASE-DEFICIENT ERYTHROCYTES [J].

GALIANO, S ;

MARENI, C ;

GAETANI, GFD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 501 (01) :1-9

[5] PYRIDINE NUCLEOTIDES IN ERYTHROCYTE METABOLISM [J].

GROSS, RT ;

SCHROEDER, EA ;

GABRIO, BW .

JOURNAL OF CLINICAL INVESTIGATION, 1966, 45 (02) :249-+

[6]

HASART E, 1970, ACTA BIOL MED GER, V24, P725

[7]

JACOBASCH G, 1974, CELLULAR MOL BIOL ER, P52

[8]

KIRKMAN HN, 1964, J LAB CLIN MED, V63, P726

[9] RED-CELL NADP+ AND NADPH IN GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENCY [J].

KIRKMAN, HN ;

GAETANI, GD ;

CLEMONS, EH ;

MARENI, C .

JOURNAL OF CLINICAL INVESTIGATION, 1975, 55 (04) :875-878

[10]

KIRKMAN HN, 1986, J BIOL CHEM, V261, P4039

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