2-OXO-HISTIDINE AS A NOVEL BIOLOGICAL MARKER FOR OXIDATIVELY MODIFIED PROTEINS

被引：113

作者：

UCHIDA, K

KAWAKISHI, S

机构：

[1] Laboratory of Food and Biodynamics, Nagoya University School of Agriculture, Nagoya

来源：

FEBS LETTERS | 1993年 / 332卷 / 03期

关键词：

2-OXO-HISTIDINE; HPLC-ECD; OXIDATIVE MODIFICATION OF PROTEIN; BIOLOGICAL MARKER;

D O I：

10.1016/0014-5793(93)80632-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a promising finding that oxidative modification of proteins by free radicals could be monitored by the formation of oxidized histidine that is detectable by reverse-phase HPLC with electrochemical detection (HPLC-ECD). When the N-protected histidine derivative (N-benzoylhistadine) was exposed to a free radical-generating system (copper/ascorbate), a number of products were detected by HPLC-ECD and the main product among them was found to be identical to N-benzoyl-2-oxo-histidine. The acid hydrolysis of N-benzoyl-2-oxo-histidine provided a single product (2-oxo-histidine) that was detected sensitively by HPLC-ECD. Thus 2-oxo-histidine was indeed generated as the main product in the oxidatively modified proteins by free radicals. Taken together, 2-oxo-histidine may be a useful biological marker for assessing protein modifications under oxidative stress.

引用

页码：208 / 210

页数：3

共 14 条

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