SEX-PHEROMONE PLASMID PAD1-ENCODED AGGREGATION SUBSTANCE OF ENTEROCOCCUS-FAECALIS IS POSITIVELY REGULATED IN TRANS BY TRAE1

被引：34

作者：

MUSCHOLL, A ^{[1
]}

GALLI, D ^{[1
]}

WANNER, G ^{[1
]}

WIRTH, R ^{[1
]}

机构：

[1] UNIV MUNICH,LEHRSTUHL MIKROBIOL,MARIA WARD STR 1A,W-8000 MUNICH 19,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 214卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb17928.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sex-pheromone-plasmid-bearing strains of Enterococcus faecalis react with sex pheromone to induce the expression of an adhesin, the so-called aggregation substance, on their cell surface. Here we show that, by complementation studies, for sex-pheromone plasmid pAD1, expression of the structural gene asa1, coding for an aggregation substance, is mediated by a diffusible factor encoded on pAD1. We were able to demonstrate that a small open reading frame, traE1, is sufficient for transcription of the operon containing asa1. A model for expression of asa1 under the influence of the positive regulator is presented, which is supported by our observation that regulation involves an all-or-nothing induction phenomenon, leading to cells either fully expressing asa1 or not at all.

引用

页码：333 / 338

页数：6

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