SUBREGIONAL MAPPING OF 13 SINGLE-COPY GENES ON THE LONG ARM OF CHROMOSOME-12 BY FLUORESCENCE INSITU HYBRIDIZATION

被引：44

作者：

MATHEW, S

MURTY, VVVS

HUNZIKER, W

CHAGANTI, RSK

机构：

[1] HOFFMANN LA ROCHE AG,BASEL,SWITZERLAND

[2] MEM SLOAN KETTERING CANC CTR,DEPT PATHOL,NEW YORK,NY 10021

来源：

GENOMICS | 1992年 / 14卷 / 03期

关键词：

D O I：

10.1016/S0888-7543(05)80184-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Subregional localization of 13 single-copy DNA sequences previously assigned to the long arm of chromosome 12 has been performed using the fluorescence in situ hybridization (FISH) technique. The following order is suggested for the 13 mapped genes: cen → COL2A1 → (VDR-D12S15) → (D12S17-D12S4-D12S14-D12S6) → D12S8 → (IAPP-MGF-D12S7-D12S12) → IGF1 → qter. Eight of the mapped genes clustered at two regions, one at 12q13 (D12S17-D12S4-D12S14-D12S6) and the other at 12q22 (IAPP-MGF-D12S7-D12S12). Our results show that single-copy DNA sequences as small as 500 bp can be successfully mapped by FISH. © 1992 Academic Press, Inc. All rights reserved.

引用

页码：775 / 779

页数：5

共 34 条

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