CONSTRUCTION OF RECOMBINATION-DEFICIENT STRAINS OF STREPTOCOCCUS-GORDONII BY DISRUPTION OF THE RECA GENE

被引：9

作者：

VICKERMAN, MM

HEATH, DG

CLEWELL, DB

机构：

[1] UNIV MICHIGAN,SCH DENT,DEPT BIOL & MAT SCI,ANN ARBOR,MI 48109

[2] UNIV MICHIGAN,SCH DENT,DEPT CARIOL & GEN DENT,ANN ARBOR,MI 48109

[3] UNIV MICHIGAN,SCH MED,DEPT MICROBIOL & IMMUNOL,ANN ARBOR,MI 48109

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 19期

关键词：

D O I：

10.1128/JB.175.19.6354-6357.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

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页码：6354 / 6357

页数：4

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