PBINPLUS - AN IMPROVED PLANT TRANSFORMATION VECTOR BASED ON PBIN19

被引：449

作者：

VANENGELEN, FA ^{[1
]}

MOLTHOFF, JW ^{[1
]}

CONNER, AJ ^{[1
]}

NAP, JP ^{[1
]}

PEREIRA, A ^{[1
]}

STIEKEMA, WJ ^{[1
]}

机构：

[1] CTR PLANT BREEDING & REPROD RES,DLO,DEPT MOLEC BIOL,6700 AA WAGENINGEN,NETHERLANDS

来源：

TRANSGENIC RESEARCH | 1995年 / 4卷 / 04期

关键词：

TRANSFORMATION VECTOR; PBIN19; TRANSGENIC PLANTS; T-DNA; AGROBACTERIUM TUMEFACIENS;

D O I：

10.1007/BF01969123

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

引用

页码：288 / 290

页数：3

共 6 条

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