TRANSFORMATION OF CORTISOL TO PREDNISOLONE BY VIABLE CELLS OF ARTHROBACTER-SIMPLEX COVALENTLY IMMOBILIZED ON CELLULOSE GRANULES

被引：10

作者：

KRYSTEVA, MA

GRIGOROVA, PM

机构：

[1] Higher Inst of Chemical Technology, Sofia, Bulg, Higher Inst of Chemical Technology, Sofia, Bulg

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1987年 / 9卷 / 09期

关键词：

BIOCHEMICAL ENGINEERING - CELL CULTURE - Applications - ENZYMES - Immobilization - MICROORGANISMS - Applications;

D O I：

10.1016/0141-0229(87)90083-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Arthrobacter simplex cells have been covalently immobilized to granules of microcrystallized regenerated cellulose by means of N-hydroxymethyl, N'-glucosylurea groups at pH 8. 5, 18 degree C and cell suspension concentration of 60 mg/ml. The immobilization yield was found to exceed 100%. The maximum initial rate of Cortisol transformation to Prednisolone remained almost constant after 20-fold transformation in a nutrient medium containing 0. 5% peptone at pH 8. 0, 32 degree C and areation with oxygen. The effect of the substrate concentration on the activity of the immobilized cells, as well as of the ratio between substrate and immobilized cells on the degree of transformation, was investigated. Microbiological observations have shown that immobilized cells can proliferate and the free cells obtained are accumulated in the nutrient medium. The immobilized cells preserve their viability for a long time when they are stored at 4 degree C.

引用

页码：538 / 541

页数：4

共 6 条

[1]

BIRNBAUM S, 1983, SOLID PHASE BIOCH, V66, P679

[2] ENZYMATIC CONVERSION OF L-HISTIDINE TO UROCANIC ACID BY WHOLE CELLS OF MICROCOCCUS-LUTEUS IMMOBILIZED ON CARBODIIMIDE ACTIVATED CARBOXYMETHYLCELLULOSE [J].