BIOLUMINESCENCE BINDING ASSAY FOR BIOTIN WITH ATTOMOLE DETECTION BASED ON RECOMBINANT AEQUORIN

被引：36

作者：

WITKOWSKI, A ^{[1
]}

RAMANATHAN, S ^{[1
]}

DAUNERT, S ^{[1
]}

机构：

[1] UNIV KENTUCKY,DEPT CHEM,LEXINGTON,KY 40506

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 11期

关键词：

D O I：

10.1021/ac00083a010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Biotinylated recombinant aequorin was used in the development of a homogeneous bioluminescence assay for biotin. It was demonstrated that the Ca2+-triggered luminescent reaction of biotinylated aequorin can be inhibited by the presence of avidin, and this inhibition io bioluminescence intensity can be used to determine the biotin concentration in a sample. The optimized assay thus developed had a detection limit for biotin of 4 amol (equivalent to 1 X 10(-14) M in the assay system), which to our knowledge is the lowest detection limit reported to date for a homogeneous competitive binding assay.

引用

页码：1837 / 1840

页数：4

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