CHEMILUMINESCENT NUCLEIC-ACID DETECTION WITH DIGOXIGENIN-LABELED PROBES - A MODEL SYSTEM WITH PROBES FOR ANGIOTENSIN CONVERTING ENZYME WHICH DETECT LESS THAN ONE ATTOMOLE OF TARGET DNA

被引：93

作者：

LANZILLO, JJ

机构：

[1] New England Medical Center, Pulmonary Division, NEMC #19, 750 Washington Street, Boston, MA 02111, United States

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90149-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Digoxigenin-labeled nucleic acid probes for angiotensin-converting enzyme were prepared by the polymerase chain reaction. In a model system, these probes were used to optimize conditions for target detection by direct chemiluminescence after dot-blot, Southern, or Northern hybridizations. Digoxigenin-labeled probes detected 23 fg (1.6 × 10-19 mol or 9 × 104 copies) of target consistently. © 1991.

引用

页码：45 / 53

页数：9

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