NUCLEOTIDE-SEQUENCE OF THE RPON (HNO) GENE REGION OF ALCALIGENES-EUTROPHUS - EVIDENCE FOR A CONSERVED GENE-CLUSTER

被引：23

作者：

WARRELMANN, J ^{[1
]}

EITINGER, M ^{[1
]}

SCHWARTZ, E ^{[1
]}

ROMERMANN, D ^{[1
]}

FRIEDRICH, B ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST PFLANZENPHYSIOL & MIKROBIOL,KONIGIN LUISE STR 12-16,W-1000 BERLIN 33,GERMANY

来源：

ARCHIVES OF MICROBIOLOGY | 1992年 / 158卷 / 02期

关键词：

RPON; SIGMA-FACTOR; HYDROGEN OXIDATION; ALCALIGENES-EUTROPHUS;

D O I：

10.1007/BF00245213

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequence of the rpoN gene, formerly designated hno, and flanking DNA regions of the aerobic hydrogen bacterium Alcaligenes eutrophus has been determined; rpoN codes for the RNA polymerase sigma factor sigma-54 involved in nitrogen regulation and diverse physiological functions of gram-negative bacteria. In A. eutrophus hydrogen metabolism is under control of rpoN. The Tn5-Mob insertion in a previously isolated pleiotropic mutant was mapped within the rpoN gene. The derived amino acid sequence of the A. eutrophus RpoN protein shows extensive homology to the RpoN proteins of other organisms. Sequencing revealed four other open reading frames: one upstream (ORF280) and three downstream (ORF130, ORF99 and ORF > 54) of the rpoN gene. A similar arrangement of homologous ORFs is found in the rpoN regions of other bacteria and is indicative of a conserved gene cluster.

引用

页码：107 / 114

页数：8

共 38 条

[1] IDENTIFICATION OF A GENE LINKED TO RHIZOBIUM-MELILOTI NTRA WHOSE PRODUCT IS HOMOLOGOUS TO A FAMILY TO ATP-BINDING PROTEINS [J].