CLONING, SEQUENCING AND EXPRESSION OF THE GENE ENCODING THE COENZYME B-12-DEPENDENT 2-METHYLENEGLUTARATE MUTASE FROM CLOSTRIDIUM-BARKERI IN ESCHERICHIA-COLI

被引：45

作者：

BEATRIX, B

ZELDER, O

LINDER, D

BUCKEL, W

机构：

[1] UNIV MARBURG,FACHBEREICH BIOL,MIKROBIOL LAB,D-35032 MARBURG,GERMANY

[2] UNIV GIESSEN,FACHBEREICHS HUMANMED,INST BIOCHEM,W-6300 GIESSEN,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 221卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1994.tb18718.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coenzyme B-12 (adenosylcobalamin)-dependent 2-methyleneglutarate mutase catalyses the carbon skeleton rearrangement of 2-methyleneglutarate to (R)-3-methylitaconate in the fermentation of nicotinic acid by the strict anaerobic bacterium Clostridium barkeri. (a) The mgm gene encoding 2-methyleneglutarate mutase was cloned and its nucleotide sequence was determined. The deduced amino acid sequence revealed a 66.8-kDa protein of 614 amino acids. It shows significant similarity in its C-terminal part to that of other cobamide-dependent enzymes. Probably, this is the coenzyme-binding region.

引用

页码：101 / 109

页数：9

共 37 条

[1] MODEL EXPERIMENTS PERTAINING TO THE MECHANISM OF ACTION OF VITAMIN-B12-DEPENDENT ALPHA-METHYLENEGLUTARATE MUTASE [J].