UPTAKE AND METABOLISM OF LONG-CHAIN 1,2-DIACYLGLYCEROLS BY RAT ADIPOCYTES AND H4IIE HEPATOMA-CELLS

The physiological long-chain diacylglycerols 1,2-dioleoylglycerol and 1,2-dimyristoylglycerol were taken up and metabolized, both by adipocytes and by hepatoma cells, in amounts sufficient to elicit biological responses, without use of emulsifier or any special cell treatment. In adipocytes the only metabolic lipid product of either diacylglycerol was triacylglycerol, In hepatoma cells both diacylglycerols were converted to a number of identified products, mainly phosphatidylcholine and triacylglycerol, This has implications for the interpretation of reports in which long-chain diacylglycerols have been found ineffective in stimulation Of cells. (C) 1995 Academic Press, Inc.