AN AUTOMATIC PULSED LASER MICROFLUOROMETER WITH HIGH SPATIAL AND TEMPORAL RESOLUTION

被引:20
作者
DOCCHIO, F
RAMPONI, R
SACCHI, CA
BOTTIROLI, G
FREITAS, I
机构
[1] POLITECN MILAN, IST FIS, CNR, CTR ELETTRON QUANTIST & STRUMENTAZIONE ELETTRON, I-20133 MILAN, ITALY
[2] UNIV PAVIA, IST ANAT COMPARATA, CNR, CTR ISTOCHIM, I-27100 PAVIA, ITALY
关键词
D O I
10.1111/j.1365-2818.1984.tb02504.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
An automatic pulsed laser microfluorometer was described with high spatial and temporal resolution. The instrument consists of: a nitrogen-laser-pumped dye-laser for the excitation of the fluorescence, a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, filters or monochromators to select the output wavelength, a fast photomultiplier tube to detect the signal, and a dual time-scale microprocessor-controlled signal averager for the acquisition and processing of the signal. The potential of the time-resolved fluorescence microscopy in studying, quantitatively and qualitatively, the properties of fluorescent molecules was given.
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页码:151 / 160
页数:10
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