OXIDATION OF THIOL IN THE VIMENTIN CYTOSKELETON

被引：22

作者：

ROGERS, KR

MORRIS, CJ

BLAKE, DR

机构：

来源：

BIOCHEMICAL JOURNAL | 1991年 / 275卷

关键词：

D O I：

10.1042/bj2750789

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.

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页码：789 / 791

页数：3

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