LOCALIZATION OF 2 GENES ENCODING PLASMA-MEMBRANE CA2+-TRANSPORTING ATPASES TO HUMAN-CHROMOSOMES 1Q25-32 AND 12Q21-23

被引：68

作者：

OLSON, S

WANG, MG

CARAFOLI, E

STREHLER, EE

MCBRIDE, OW

机构：

[1] NCI,BIOCHEM LAB,BETHESDA,MD 20892

[2] SWISS FED INST TECHNOL,BIOCHEM LAB,CH-8092 ZURICH,SWITZERLAND

来源：

GENOMICS | 1991年 / 9卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0888-7543(91)90356-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human plasma membrane Ca2+-ATPase (PMCA) isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. Cloned cDNAs for two of these isoforms have been used as probes to localize chromosomally the human PMCA1 (ATP2B1) gene to 12q21-q23 and PMCA4 (ATP2B2) to 1q25-q32. These results were obtained by three independent methods, including Southern analysis of human-rodent somatic cell hybrids, in situ hybridization of human metaphase spreads, and genetic linkage analysis in the CEPH pedigrees. High-frequency RFLPs detected at each locus were used in these linkage analyses. No evidence was obtained for multiple copies of the gene at either locus. A cross-hybridizing sequence was detected with PMCA4 probes on Xq13-qter at low stringency. Further studies are required to determine whether this X-chromosomal sequence represents a third member of the PMCA gene family. © 1991.

引用

页码：629 / 641

页数：13

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