Lipo-oligosaccharide of Campylobacter lari strain PC 637. Structure of the liberated oligosaccharide and an associated extracellular polysaccharide

被引：23

作者：

Aspinall, GO

Monteiro, MA

Pang, HP

Kurjanczyk, LA

Penner, JL

机构：

[1] UNIV TORONTO,DEPT MOLEC BIOL & MED GENET,CARBOHYDRATE RES CTR,TORONTO,ON M5S 1A5,CANADA

[2] UNIV TORONTO,DEPT MICROBIOL,TORONTO,ON M5S 1A5,CANADA

来源：

CARBOHYDRATE RESEARCH | 1995年 / 279卷

基金：

加拿大自然科学与工程研究理事会; 英国医学研究理事会;

关键词：

Campylobacter lari PC 637; lipo-oligosaccharide; extracellular polysaccharide; tetraglycosyl phosphate polymer;

D O I：

10.1016/0008-6215(95)00283-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipo-oligosaccharide from phenol-water extraction of cells of Campylobacter lari strain PC 637 was separated as a water-insoluble gel of low relative molecular mass (M(r)) from a water-soluble extracellular polysaccharide of high M(r). Structural investigations were performed on the lipo-oligosaccharide and the extracellular polysaccharide, variously using H-1, C-13, and P-31 NMR spectroscopy, linkage analysis, and fast atom bombardment-mass spectrometry of permethylated derivatives of the glycans and their products of chemical and enzymic degradation. The following structures are proposed for the highly branched oligosaccharide region: GRAPHICS and for the tetraglycosyl phosphate repeating unit of the extracellular polysaccharide: GRAPHICS GRAPHICS

引用

页码：227 / 244

页数：18

共 28 条

[1] CHEMICAL-STRUCTURE OF THE CORE REGION OF CAMPYLOBACTER-JEJUNI SEROTYPE O-2 LIPOPOLYSACCHARIDE [J].