THE QUALITATIVE AND QUANTITATIVE-ANALYSIS OF INSECT HEMOLYMPH SUGARS BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

1. 1. A procedure is described for the analysis of sugars in insect hemolymph by high performance thin-layer chromatography. 2. 2. Densitometric scanning of spots after plate developments shows linear responses with most sugars in an analytical range of 125ng-2.0 /gmg; detection limits range from 30/2-60 ng. 3. 3. Quantitative measurements of trehalose, glucose and fructose can be made on hemolymph sample volumes of less than one microliter, allowing blood sugar analysis of individual insects. 4. 4. Hemolymph sugar determinations on six species of insects agree well with values reported in the literature, but mean values for a species often showed higher variability because samples were taken from individuals. © 1990.