BARRIER FUNCTION REGULATES EPIDERMAL DNA-SYNTHESIS

被引:193
作者
PROKSCH, E
FEINGOLD, KR
MAN, MQ
ELIAS, PM
机构
[1] VET ADM MED CTR,DERMATOL SERV 190,4150 CLEMENT ST,SAN FRANCISCO,CA 94121
[2] VET ADM MED CTR,MED SERV,SAN FRANCISCO,CA 94121
[3] UNIV CALIF SAN FRANCISCO,SCH MED,SAN FRANCISCO,CA 94143
[4] UNIV GOTTINGEN,DERMATOL CLIN,W-3400 GOTTINGEN,GERMANY
关键词
PERMEABILITY BARRIER; TRANSCUTANEOUS WATER LOSS; EPIDERMAL HYPERPLASIA; EPIDERMAL DNA SYNTHESIS;
D O I
10.1172/JCI115183
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We examined the possibility that the cutaneous permeability barrier regulates epidermal DNA synthesis in two acute and two chronic models of barrier perturbation. In animals treated topically with acetone, DNA synthesis is increased 102%, in tape-stripped animals 127%, in essential fatty acid deficient animals 50%, and in animals chronically treated with topical lovastatin 64%. This linkage between disturbances in barrier function and increased DNA synthesis is further supported by specific and correlative observations: (a) in these disparate models, artificial replacement of the barrier with a water-impermeable membrane inhibits the expected increase in DNA synthesis; (b) the extent of the burst in DNA synthesis is proportional to the degree of barrier abrogation; (c) the inhibition of DNA synthesis by membranes is directly related to the degree of permeability of these occlusive membranes, i.e., the more impermeable the greater the degree of inhibition; (d) topical treatment with lipids that restore barrier function corrects the increase in DNA synthesis; and (e) barrier abrogation with acetone produces an increase in epidermal DNA synthesis without altering bulk protein synthetic rates in contrast to events known to follow injury or cell replacement. Autoradiographic studies show that the increase in DNA synthesis after acetone treatment is limited to the epidermal basal layer. This constellation of findings strongly suggests that cutaneous barrier function is one factor that regulates epidermal DNA synthesis.
引用
收藏
页码:1668 / 1673
页数:6
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