A TECHNIQUE FOR DETECTING ANTIFUNGAL ACTIVITY OF PROTEINS SEPARATED BY POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：17

作者：

DEBOLLE, MFC ^{[1
]}

GODERIS, IJ ^{[1
]}

TERRAS, FRG ^{[1
]}

CAMMUE, BPA ^{[1
]}

BROEKAERT, WF ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,W DE CROYLAAN 42,B-3001 HEVERLEE,BELGIUM

来源：

ELECTROPHORESIS | 1991年 / 12卷 / 06期

关键词：

D O I：

10.1002/elps.1150120612

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the polyacrylamide gel. The position of proteins with antifungal activity can be determined on a diffusion blot prepared from the same gel. The technique is illustrated for three antifungal plant proteins, i.e. a-purothionin, Urtica dioica agglutinin, and tobacco chitinase.

引用

页码：442 / 444

页数：3

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