ISOLATION AND SEQUENCE OF A FULL-LENGTH CDNA-ENCODING A NOVEL RAT INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE

被引：29

作者：

THOMAS, S

BRAKE, B

LUZIO, JP

STANLEY, K

BANTING, G

机构：

[1] UNIV BRISTOL,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

[2] INST ARZENEMITTEL,BERLIN,GERMANY

[3] UNIV CAMBRIDGE,DEPT CLIN BIOCHEM,CAMBRIDGE,ENGLAND

[4] HEART RES INST,SYDNEY,NSW,AUSTRALIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1220卷 / 02期

关键词：

IP3; 3-KINASE; CDNA; NUCLEOTIDE SEQUENCE; PCR; (RAT);

D O I：

10.1016/0167-4889(94)90139-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoscreening a rat liver cDNA expression library has led to the isolation of a full-length cDNA clone encoding a novel isoform of rat inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase). Sequence comparison shows it (i) to be 93% identical to human hippocampus IP3 3-kinase B over 468 residues at the protein level, and (ii) to encode a protein 204 amino acids larger than the published sequence of its human homologue.

引用

页码：219 / 222

页数：4

共 12 条

[1]

Ausubel FM, 1989, SHORT PROTOCOLS MOL

[2]

BANTING G, 1989, FEBS LETT, V254, P171

[3] PUEX, A BACTERIAL EXPRESSION VECTOR RELATED TO PEX WITH UNIVERSAL HOST SPECIFICITY [J].