REGENERATION OF BOVINE PANCREATIC RIBONUCLEASE-A .4. TEMPERATURE-DEPENDENCE OF THE REGENERATION RATE

The rate of regeneration of bovine pancreatic ribonuclease A with oxidized and reduced dithiothreitol (DTT(ox) and DTT(red), respectively) decreases by a factor of 10 when the temperature is increased from 25 to 37-degrees-C. The rate of regeneration of RNase A with oxidized and reduced glutathione increases slightly over that same range of temperature. This suggests that the regeneration processes with the two types of redox reagents proceed through different pathways. There is a significant change in the distribution of three-disulfide intermediates populated during regeneration with DTT(ox)/DTT(red) over the range of temperature 15-37-degrees-C that suggests that the three-disulfide species populated at 15-degrees-C are directly involved in the major regeneration pathway observed at 25-degrees-C.