Mammalian expression of single chain variable region fragments dimerized by Fc regions

被引：4

作者：

Kato, T

Sato, K

Suzuki, S

Sasakawa, T

Kurokawa, M

Nishioka, K

Yamamoto, K

机构：

[1] SCI UNIV TOKYO,DEPT BIOTECHNOL,CHIBA,JAPAN

[2] MITSUBISHI KAGAKU BIOCLIN LABS INC,TOKYO,JAPAN

[3] FUKUSHIMA MED COLL,DEPT INTERNAL MED 2,FUKUSHIMA,JAPAN

来源：

MOLECULAR BIOLOGY REPORTS | 1995年 / 21卷 / 03期

关键词：

Fc region; single chain variable region fragment; immunoglobulin;

D O I：

10.1007/BF00997236

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To promote application of a single chain variable region fragment (sFv) in immunoglobulins, a sFv gene was connected to an IgG1 Fc gene, designated as a sFvc gene, and used for transfection of Sp2/0. As a result, the sFvc protein was found to be secreted in a dimeric form. It is thus felt that the sFvc protein, which mimicks the shape of a naturally occurring antibody, can be simple and useful to reproduce divalency and Fc-associated effecter functions as seen in a natural antibody.

引用

收藏

页码：141 / 146

页数：6

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