SEQUENCE AND EVOLUTION OF THE GENE FOR THE MONOMERIC GLOBIN-I AND ITS LINKAGE TO GENES-CODING FOR DIMERIC GLOBINS IN THE INSECT CHIRONOMUS-THUMMI

被引：13

作者：

KAO, WY

HANKELN, T

SCHMIDT, ER

BERGTROM, G

机构：

[1] UNIV WISCONSIN, DEPT BIOL SCI, MILWAUKEE, WI 53201 USA

[2] UNIV MAINZ, INST GENET MOLEK GENET, D-55099 MAINZ, GERMANY

来源：

JOURNAL OF MOLECULAR EVOLUTION | 1995年 / 40卷 / 04期

关键词：

INSECT GLOBIN GENES; MOLECULAR EVOLUTION; ALLELES; IN SITU HYBRIDIZATION;

D O I：

10.1007/BF00164021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated genomic clones containing sequences encoding globins I and IA from a Chironomus thummi thummi genomic library. Three clones contain globin IA (ctt-1A) genes, while one contains a globin I (ctt-1) gene. The coding regions of the four genes are identical except for the single base substitution accounting for the, globin I/IA polymorphism. The noncoding DNA flanking the coding region is more than 98% similar, confirming a previous hypothesis that the globin ctt-1 and ctt-1A genes are alleles. Hemoglobins I and IA are monomeric in the insect hemolymph. Earlier in situ hybridization studies suggested that monomeric and dimeric globin genes are clustered at different chromosomal loci. In situ hybridization of ctt-1 DNA to polytene salivary gland chromosomes places the ctt-1 gene on the same band as genes for the dimeric globins II beta and VIIB, forcing revision of the earlier hypothesis that genes for monomeric and dimeric globin genes are at different loci. The evolution of the ctt-1 and ctt-1A alleles and of the two globin gene loci are discussed.

引用

页码：354 / 361

页数：8

共 44 条

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