PITUITARY ADENYLATE CYCLASE-ACTIVATING PEPTIDE STIMULATES NEURITE GROWTH IN PC12 CELLS

被引：56

作者：

HERNANDEZ, A ^{[1
]}

KIMBALL, B ^{[1
]}

ROMANCHUK, G ^{[1
]}

MULHOLLAND, MW ^{[1
]}

机构：

[1] UNIV MICHIGAN,CTR GASTROINTESTINAL PEPTIDE,DEPT SURG,ANN ARBOR,MI 48109

来源：

PEPTIDES | 1995年 / 16卷 / 05期

关键词：

PACAP; NEURITE GROWTH; PC12; CELLS;

D O I：

10.1016/0196-9781(95)00059-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of PACAP-38 to stimulate morphological development was studied using rat pheochromocytoma PC12 cells. PACAP-38 produced concentration-dependent increases in percentage of cells exhibiting neurite extension. Similar increases were produced by forskolin (28 +/- 2% at 96 h) and 8-bromo cAMP (30 +/- 2%). Vasoactive intestinal peptide and alpha-calcitonin gene-related peptide were without effect. PACAP-38 produced significant increases in PC12 cell cAMP content and inositol phosphate turnover. Intracellular [Ca2+] increased from 169 +/- 14 nM to 560 +/- 58 nM in response to 1 mu M PACAP-38. PACAP-stimulated neurite outgrowth was abolished by R(p)cAMPS, an inhibitor of cAMP-dependent kinases but was unaffected by the protein kinase C antagonist 117.

引用

页码：927 / 932

页数：6

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