PURIFICATION AND PROPERTIES OF NEWT PROLACTIN

被引：25

作者：

MATSUDA, K ^{[1
]}

YAMAMOTO, K ^{[1
]}

KIKUYAMA, S ^{[1
]}

机构：

[1] WASEDA UNIV, SCH EDUC, DEPT BIOL, SHINJUKU KU, TOKYO 169, JAPAN

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 1990年 / 77卷 / 01期

关键词：

D O I：

10.1016/0016-6480(90)90206-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A highly purified prolactin (PRL) was obtained from pituitary glands of newts, Cynops pyrrhogaster, by extraction of acetone-dried powder with acid acetone and high-performance liquid chromatography (HPLC) on Mono-Q (anion exchange), Superose-12 (gel filtration), and TSK-gel ODS-120T (reverse-phase) columns with a yield of 4.5 mg per 325 mg of protein starting material. Purification was monitored by SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting analysis employing antiserum against bullfrog PRL. Newt PRL thus obtained has a molecular weight of 23,000 as determined by SDS-PAGE. The isoelectric point is 4.7 as determined by isoelectric focusing. The amino acid composition closely resembles that of anuran PRLs. This hormone was as potent as bovine PRL in stimulating collagen synthesis in the bullfrog tadpole tail fin. Antiserum against newt PRL was produced by immunizing a rabbit. Histological studies on newt adeno-hypophyses revealed that the cells that immunologically reacted with the antiserum against newt PRL correspond to the ones positively stained with the antiserum against bullfrog PRL. © 1990.

引用

页码：63 / 69

页数：7

共 22 条

[1]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[2]

ENSOR DM, 1978, COMP ENDOCRINOLOGY P, P45

[3]

GOUDIE RB, 1966, LANCET, V2, P1224

[4]

Gray W. R., 1967, METHOD ENZYMOL, V11, P469

[5]

KIKUYAMA S, 1988, INT CONGR SER, V819, P359

[6]

KIKUYAMA S, 1980, GUNMA S ENDOCRINOL, V17, P3

[7]

Li C H, 1970, Arch Biochem Biophys, V141, P705, DOI 10.1016/0003-9861(70)90191-8

[8]

LI CH, 1980, CHEM PROLACTIN HORMO, V8, P2

[9]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[10]

Nicoll C.S., 1972, LACTOGENIC HORMONES, P299

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