VECTOR METHYLATION INHIBITS TRANSCRIPTION FROM THE SV40 EARLY PROMOTER

被引：20

作者：

BRYANS, M ^{[1
]}

KASS, S ^{[1
]}

SEIVWRIGHT, C ^{[1
]}

ADAMS, RLP ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND

来源：

FEBS LETTERS | 1992年 / 309卷 / 01期

基金：

英国惠康基金;

关键词：

DNA METHYLATION; SV40; PROMOTER; CPG ISLAND; SP1; CHROMATIN STRUCTURE;

D O I：

10.1016/0014-5793(92)80748-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methylation of a plasmid containing the SV40 promoter linked to the chloramphenicol acetyl transferase (CAT) gene. with either murine DNA methylase or methylase SssI results in inhibition of the expression of the reporter gene after transfection into cultured cells. Methylation of the plasmid with the methylases HhaI and HpaII has no effect on the expression of this gene. Protein-DNA interactions in the SV40 promoter are not affected by the presence of methylcytosine suggesting that inactivation results from the formation of an inactive chromatin structure that is dependent on the high CG content of the plasmid.

引用

页码：97 / 102

页数：6

共 29 条

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