DNA-SEQUENCE MAPPING BY FLUORESCENCE INSITU HYBRIDIZATION

被引：12

作者：

BRANDRIFF, BF

GORDON, LA

TRASK, BJ

机构：

[1] Lawrence Livermore National Laboratory, Biomedical Sciences Division, University of California, Livermore, California

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1991年 / 18卷 / 04期

关键词：

DNA PROBES; CHROMATIN TARGETS; MOLECULAR BIOLOGY TECHNIQUES; CLASSICAL CYTOGENICS;

D O I：

10.1002/em.2850180410

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Various types of DNA probes, such as total genomic DNA, repetitive sequences, unique sequences, and composites of chromosome-specific DNA probes, can be used with fluorescence in situ hybridization (FISH) techniques to address research questions having to do with localization, mapping, and distribution of DNA in situ. FISH involves the formation of a hetero-duplex between such DNA probes and chromatin targets on a microscope slide, which can be visualized with fluorescent reporter molecules. Three chromatin targets-metaphase chromosomes, somatic interphases, and zygote interphases-offer increasingly extended states of chromatin which can be strategically selected, individually or in combination, to address specific research questions of interest.

引用

页码：259 / 262

页数：4

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