1,25-DIHYDROXYVITAMIN-D-3 DESTABILIZES C-MYC MESSENGER-RNA IN HL-60 LEUKEMIC-CELLS

被引：19

作者：

MANGASARIAN, K ^{[1
]}

MELLON, WS ^{[1
]}

机构：

[1] UNIV WISCONSIN,SCH PHARM,425 N CHARTER ST,MADISON,WI 53706

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1172卷 / 1-2期

关键词：

1,25-DIHYDROXYVITAMIN-D-3; MESSENGER RNA STABILITY; C-MYC; HL-60;

D O I：

10.1016/0167-4781(93)90269-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The differentiation process is accompanied by alterations in the expression of a variety of genes. Monocytic maturation of hematopoietic cells (HL-60) induced by 1,25-dihydroxyvitamin D-3 (1,25(OH)2D3), results in a decrease in steady state c-myc mRNA levels. To elucidate the mechanism by which 1,25(OH)2D3 regulates c-myc mRNA expression, transcriptional and post-transcriptional modes of regulation were investigated. No transcriptional regulation was identified, however, 1,25(OH)2D3 appeared to decrease steady state c-myc mRNA levels by increasing its turnover rate. Using actinomycin D to block transcription, the half-life of c-myc mRNA was shown to decrease from 20 min in the absence of 1,25(OH)2D3 to < 5 min in the presence of 1,25(OH)2D3. Cycloheximide reversed the instability induced by 1,25(OH)2D3, prolonging the half-life of c-myc mRNA in both uninduced and 1,25(OH)2D3-induced HL-60 cells to > 60 min, indicating a translational requirement for the destabilization process. Additionally, the c-myc mRNA instability induced by 1,25(OH)2D3 in HL-60 appears to be a specific result of this agent, as indicated by the inability of other monocytic and granulocytic differentiation inducing agents to destabilize c-myc mRNA.

引用

页码：55 / 63

页数：9

共 39 条

[1]

ATWATER JA, 1990, ANNU REV GENET, V24, P519

[2] INDUCTION OF MONOCYTIC DIFFERENTIATION AND BONE-RESORPTION BY 1,25-DIHYDROXYVITAMIN-D3 [J].

BARSHAVIT, Z ;

TEITELBAUM, SL ;

REITSMA, P ;

HALL, A ;

PEGG, LE ;

TRIAL, J ;

KAHN, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (19) :5907-5911

[3] A BLOCK TO ELONGATION IS LARGELY RESPONSIBLE FOR DECREASED TRANSCRIPTION OF C-MYC IN DIFFERENTIATED HL60 CELLS [J].

BENTLEY, DL ;

GROUDINE, M .

NATURE, 1986, 321 (6071) :702-706

[4] C-MYC GENE IS TRANSCRIBED AT HIGH-RATE IN G0-ARRESTED FIBROBLASTS AND IS POST-TRANSCRIPTIONALLY REGULATED IN RESPONSE TO GROWTH-FACTORS [J].

BLANCHARD, JM ;

PIECHACZYK, M ;

DANI, C ;

CHAMBARD, JC ;

FRANCHI, A ;

POUYSSEGUR, J ;

JEANTEUR, P .

NATURE, 1985, 317 (6036) :443-445

[5] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[6] CHARACTERIZATION OF THE TRANSCRIPTION PRODUCTS OF GLYCERALDEHYDE 3-PHOSPHATE-DEHYDROGENASE GENE IN HELA-CELLS [J].

DANI, C ;

PIECHACZYK, M ;

AUDIGIER, Y ;

ELSABOUTY, S ;

CATHALA, G ;

MARTY, L ;

FORT, P ;

BLANCHARD, JM ;

JEANTEUR, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 145 (02) :299-304

[7] INCREASED RATE OF DEGRADATION OF C-MYC MESSENGER-RNA IN INTERFERON-TREATED DAUDI CELLS [J].

DANI, C ;

MECHTI, N ;

PIECHACZYK, M ;

LEBLEU, B ;

JEANTEUR, P ;

BLANCHARD, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :4896-4899

[8] C-MYC REGULATION DURING RETINOIC ACID-INDUCED DIFFERENTIATION OF F9 CELLS IS POSTTRANSCRIPTIONAL AND ASSOCIATED WITH GROWTH ARREST [J].

DEAN, M ;

LEVINE, RA ;

CAMPISI, J .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (02) :518-524

[9] DNA-SEQUENCES IN THE RAT OSTEOCALCIN GENE THAT BIND THE 1,25-DIHYDROXYVITAMIN-D3 RECEPTOR AND CONFER RESPONSIVENESS TO 1,25-DIHYDROXYVITAMIN-D3 [J].

DEMAY, MB ;

GERARDI, JM ;

DELUCA, HF ;

KRONENBERG, HM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :369-373

[10]

DEMAY MB, 1989, J BIOL CHEM, V264, P2279

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