ISOLATION OF DICTYOSTELIUM-DISCOIDEUM CYTOKINESIS MUTANTS BY RESTRICTION ENZYME-MEDIATED INTEGRATION OF THE BLASTICIDIN-S RESISTANCE MARKER

被引：157

作者：

ADACHI, H ^{[1
]}

HASEBE, T ^{[1
]}

YOSHINAGA, K ^{[1
]}

OHTA, T ^{[1
]}

SUTOH, K ^{[1
]}

机构：

[1] KOGAKUIN UNIV,FAC ENGN,DEPT APPL CHEM,SHINJUKU KU,TOKYO 16391,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2880

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an improved REMI (restriction enzyme-mediated integration) system for generating mutant Dictyostelium cells quickly and efficiently for systematic screening of cytokinesis mutants. By means of this system, three cytokinesis mutants that grow as giant and multinucleate cells were isolated from 2,000 Dictyostelium transformants. Southern blot analysis of these mutants revealed that a single copy of the tag DNA was integrated into each genome. The tag with flanking genomic DNA at both ends was rescued from one of the mutants and reintroduced into the parental Ax2 strain. Homologous recombination of the rescued gene and the Dictyostelium genome led to the phenotypical changes expected for cytokinesis mutants. (C) 1994 Academic Press, Inc.

引用

页码：1808 / 1814

页数：7

共 17 条

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