MUTATIONS OF 2 LYSINE RESIDUES IN THE CDR LOOPS OF A RECOMBINANT IMMUNOTOXIN THAT REDUCE ITS SENSITIVITY TO CHEMICAL DERIVATIZATION

被引：12

作者：

BENHAR, I ^{[1
]}

BRINKMANN, U ^{[1
]}

WEBBER, KO ^{[1
]}

PASTAN, I ^{[1
]}

机构：

[1] NCI,DIV CANC BIOL DIAGN & CTR,MOLEC BIOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892

来源：

BIOCONJUGATE CHEMISTRY | 1994年 / 5卷 / 04期

关键词：

D O I：

10.1021/bc00028a007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

B3(Fv)-PE38 is a recombinant single-chain immunotoxin in which the Fv region of monoclonal antibody B3 is connected to a truncated form of Pseudomonas exotoxin. It would be desirable to use the lysine residues of the molecule for chemical modification so that it can be derivatized with poly(ethylene glycol) to achieve reduced immunogenicity or with the Bolton-Hunter reagent for biodistribution studies. We found that derivatizing lysine residues of B3(Fv)-PE38 causes a marked loss of specific target cell cytotoxicity and/or immunoreactivity. Here we show that two lysine residues in the antibody-combining region of B3(Fv)-PE38 can be replaced with arginines, with only a small loss of cytotoxicity and no change in specificity. This mutant molecule is 3-fold more resistant to inactivation by derivatization with succinimidyl 4-(N-maleimidomethyl)cyclohexanel-carboxylate (SMCC) or Bolton-Hunter reagent.

引用

页码：321 / 326

页数：6

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