PREPARATION AND USE OF POLYPHOSPHATE-MODIFIED ZIRCONIA FOR PURIFICATION OF NUCLEIC-ACIDS AND PROTEINS

被引：28

作者：

LORENZ, B ^{[1
]}

MARME, S ^{[1
]}

MULLER, WEG ^{[1
]}

UNGER, K ^{[1
]}

SCHRODER, HC ^{[1
]}

机构：

[1] UNIV MAINZ,INST ANORGAN CHEM & ANALYT CHEM,D-55099 MAINZ,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 216卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyphosphates of chain lengths between 3 and 100 were coupled to porous zirconia particles and used for affinity chromatography of nucleic acids and proteins. It is demonstrated that single-stranded RNA and DNA are strongly retained on the polyphosphate-modified zirconia support, while double-stranded DNA is only weakly bound if at all. Protein separation on this packing can be performed by applying both high-performance liquid chromatography and batch procedures. The results show that polyphosphate-modified zirconia is a useful affinity packing for immobilization and purification of biomacromolecules. © 1994 Academic Press, Inc.

引用

页码：118 / 126

页数：9

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