CHARACTERIZATION OF 3 MEMBERS OF THE ACC SYNTHASE GENE FAMILY IN SOLANUM-TUBEROSUM L

被引:43
作者
DESTEFANOBELTRAN, LJC [1 ]
VANCAENEGHEM, W [1 ]
GIELEN, J [1 ]
RICHARD, L [1 ]
VAN MONTAGU, M [1 ]
VAN DER STRAETEN, D [1 ]
机构
[1] STATE UNIV GHENT, GENET LAB, B-9000 GHENT, BELGIUM
来源
MOLECULAR AND GENERAL GENETICS | 1995年 / 246卷 / 04期
关键词
ACC SYNTHASE; AUXIN; ETHYLENE; SOLANUM TUBEROSUM; WOUNDING;
D O I
10.1007/BF00290453
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two genomic clones corresponding to three members of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene family in potato (Solanum tuberosum L.) have been isolated and sequenced. Two highly homologous genes, ST-ACS1A and ST-ACS1B, transcribed in opposite directions were found in an 8.9 kb region. Their coding sequences are interrupted by two introns at identical positions. Their closest relative in tomato is the LE-ACS3 gene. The third gene in potato, ST-ACS2, was found in a 4 kb region and shows a gene structure similar to that of the tomato LE-ACS4 gene and to the mung bean VR-ACS4 and VR-ACS5 genes. Based on its lack of significant homology to the tomato gene family and its closeness to the VR-ACS4 and VR-ACS5 genes, we propose that LE-ACS7 represents an additional isoform in the tomato genome. Moreover, in a phylogenetic comparison of known ACC synthases, the ST-ACS2 isoform was grouped in a separate lineage together with the mung bean VR-ACS4 and VR-ACS5, and the moth orchid DS-ACS1A and DS-ACS1B gene products. Expression of the three potato genes was studied by reverse transcription-polymerase chain reaction on total RNA. The twin genes are positively regulated by indole-3-acetic acid in hypocotyls and expression is modulated by wounding in the leaves. The third gene is responsive to ethylene and wounding mainly in tubers. The roles of these three genes and of other members of the ACC synthase gene family in vegetative processes of potato such as tuberization, dormancy, and sprouting have yet to be determined.
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收藏
页码:496 / 508
页数:13
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