IDENTIFICATION OF EIMERIA-BOVIS MEROZOITE CDNAS USING DIFFERENTIAL MESSENGER-RNA DISPLAY

被引：17

作者：

ABRAHAMSEN, MS ^{[1
]}

JOHNSON, RR ^{[1
]}

HATHAWAY, M ^{[1
]}

WHITE, MW ^{[1
]}

机构：

[1] MONTANA STATE UNIV,BOZEMAN,MT 59717

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1995年 / 71卷 / 02期

关键词：

EIMERIA BOVIS; APICOMPLEXA; CDNA; DEVELOPMENTAL REGULATION; POLYMERASE CHAIN REACTION; DIFFERENTIAL MESSENGER-RNA DISPLAY;

D O I：

10.1016/0166-6851(94)00052-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differences in gene expression between Eimeria bovis sporozoites and first-generation merozoites were analyzed using the technique of differential mRNA display. Approx. 5% of the sequences detected in first-generation merozoites appear to be unique relative to sporozoites. Several of the bands corresponding to merozoite-specific gene expression were isolated and cloned. Northern blot analysis revealed that the cDNA fragments DMZ-7, DMZ-8 and NMZ-6 hybridized to mRNAs expressed at > 50-fold higher levels in merozoites relative to sporozoites. A fourth cDNA fragment, NMZ-4, hybridized to a mRNA expressed at 3-fold higher levels in merozoites. Further characterization demonstrated that expression of DMZ-8 in E. bovis-infected bovine cells begins as early as 12 h after sporozoite invasion and continues throughout the entire 14 days of first-generation schizogony. Sequence analysis of each of the four merozoite cDNAs failed to identify any significant similarity to any entries in the GenBank database, suggesting that these developmentally regulated genes may be unique to coccidian parasites.

引用

页码：183 / 191

页数：9

共 28 条

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