EFFICIENT TRANSFORMATION OF AGROBACTERIUM-TUMEFACIENS BY ELECTROPORATION

被引：133

作者：

MERSEREAU, M

PAZOUR, GJ

DAS, A

机构：

[1] UNIV MINNESOTA,DEPT BIOCHEM,1479 GORTNER AVE,ST PAUL,MN 55108

[2] UNIV MINNESOTA,INST PLANT MOLEC GENET,ST PAUL,MN 55108

来源：

GENE | 1990年 / 90卷 / 01期

关键词：

methylation; phytopathogen; Recombinant DNA; restriction system; soil bacteria; Ti plasmid;

D O I：

10.1016/0378-1119(90)90452-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 108 transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens. © 1990.

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页码：149 / 151

页数：3

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