GLYCEROL-INDUCED UNRAVELING OF THE TIGHT HELICAL CONFORMATION OF ESCHERICHIA-COLI TYPE-1 FIMBRIAE

被引：16

作者：

ABRAHAM, SN

LAND, M

PONNIAH, S

ENDRES, R

HASTY, DL

BABU, JP

机构：

[1] UNIV TENNESSEE CTR HLTH SCI,DEPT ANAT & NEUROSCI,MEMPHIS,TN 38104

[2] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110

[3] UNIV TENNESSEE CTR HLTH SCI,DEPT MED & MICROBIOL,MEMPHIS,TN 38104

[4] JEWISH HOSP ST LOUIS,DEPT PATHOL,ST LOUIS,MO 63110

[5] UNIV TENNESSEE CTR HLTH SCI,DENT RES CTR,MEMPHIS,TN 38104

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 15期

关键词：

D O I：

10.1128/JB.174.15.5145-5148.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-binding activity and trypsin sensitivity than native fimbriae. Removal of glycerol by dialysis results in spontaneous reassembly of the linear polymers into structures morphologically, antigenically, and functionally indistinguishable from native fimbriae.

引用

页码：5145 / 5148

页数：4

共 14 条

[1] ABRAHAM S, 1988, INFECT IMMUN, V169, P1023
[2] ASSEMBLY OF A CHEMICALLY SYNTHESIZED PEPTIDE OF ESCHERICHIA-COLI TYPE-1 FIMBRIAE INTO FIMBRIA-LIKE ANTIGENIC STRUCTURES
ABRAHAM, SN
BEACHEY, EH
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (06) : 2460 - 2465
[3] IDENTIFICATION OF 2 ANCILLARY SUBUNITS OF ESCHERICHIA-COLI TYPE-1 FIMBRIAE BY USING ANTIBODIES AGAINST SYNTHETIC OLIGOPEPTIDES OF FIM GENE-PRODUCTS
ABRAHAM, SN
GOGUEN, JD
SUN, D
KLEMM, P
BEACHEY, EH
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (12) : 5530 - 5536
[4] CONSERVATION OF THE D-MANNOSE-ADHESION PROTEIN AMONG TYPE-1 FIMBRIATED MEMBERS OF THE FAMILY ENTEROBACTERIACEAE
ABRAHAM, SN
SUN, DX
DALE, JB
BEACHEY, EH
[J]. NATURE, 1988, 336 (6200) : 682 - 684
[5] ABRAHAM SN, 1983, J EXP MED, V158, P1128
[6] STRUCTURE FUNCTION SYNTHESIS AND GENETIC CONTROL OF BACTERIAL PILI AND A MOLECULAR MODEL FOR DNA AND RNA TRANSPORT IN GRAM NEGATIVE BACTERIA
BRINTON, CC
[J]. TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES, 1965, 27 (08): : 1003 - &
[7] ANTIGENIC QUANTITATION OF TYPE-1 FIMBRIAE ON THE SURFACE OF ESCHERICHIA-COLI-CELLS BY AN ENZYME-LINKED IMMUNOSORBENT INHIBITION ASSAY
DODD, DC
EISENSTEIN, BI
[J]. INFECTION AND IMMUNITY, 1982, 38 (02) : 764 - 773
[8] DISSOCIATION AND REASSEMBLY OF ESCHERICHIA-COLI TYPE 1 PILI
ESHDAT, Y
SILVERBLATT, FJ
SHARON, N
[J]. JOURNAL OF BACTERIOLOGY, 1981, 148 (01) : 308 - 314
[9] IDENTIFICATION AND CHARACTERIZATION OF ESCHERICHIA-COLI TYPE-1 PILUS TIP ADHESION PROTEIN
HANSON, MS
BRINTON, CC
[J]. NATURE, 1988, 332 (6161) : 265 - 268
[10] 3 FIM GENES REQUIRED FOR THE REGULATION OF LENGTH AND MEDIATION OF ADHESION OF ESCHERICHIA-COLI TYPE-1 FIMBRIAE
KLEMM, P
CHRISTIANSEN, G
[J]. MOLECULAR & GENERAL GENETICS, 1987, 208 (03): : 439 - 445

← 1 2 →