RENATURATION AND PARTIAL PEPTIDE SEQUENCING OF MITOGEN-ACTIVATED PROTEIN-KINASE (MAP KINASE) ACTIVATOR FROM RABBIT SKELETAL-MUSCLE

被引：50

作者：

WU, J

MICHEL, H

ROSSOMANDO, A

HAYSTEAD, T

SHABANOWITZ, J

HUNT, DF

STURGILL, TW

机构：

[1] UNIV VIRGINIA,DEPT MED,BOX 419,CHARLOTTESVILLE,VA 22908

[2] UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908

[3] UNIV VIRGINIA,DEPT CHEM,CHARLOTTESVILLE,VA 22908

来源：

BIOCHEMICAL JOURNAL | 1992年 / 285卷

关键词：

D O I：

10.1042/bj2850701

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitogen-activated protein kinase (MAP kinase) activator was purified 2000-fold from skeletal muscle, and proteins which co-purified with the activator were analysed after SDS/PAGE by renaturation and partial sequencing. Activity for tyrosine and threonine phosphorylation of MAP kinase was present in two bands of approx. 48 and 46 kDa, which have sequence similarity to small GTP-binding protein p25 GDP dissociation inhibitor and protein kinases (PBS2, SPK 1+, STE7, BYR1) respectively.

引用

页码：701 / 705

页数：5

共 23 条

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