CHROMATOGRAPHIC TANK DESIGNED TO OBTAIN HIGHLY REPRODUCIBLE HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAMS OF GANGLIOSIDES AND NEUTRAL GLYCOSPHINGOLIPIDS

被引:55
作者
NORES, GA [1 ]
MIZUTAMARI, RK [1 ]
KREMER, DM [1 ]
机构
[1] CONSEJO NACL INVEST CIENT & TECN,CIQUIBIC,RA-5016 CORDOBA,ARGENTINA
关键词
D O I
10.1016/S0021-9673(94)89015-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Although high-performance thin-layer chromatography (HPTLC) is one of the most useful methods in glycosphingolipid studies, there are some difficulties with the reproducibility of the chromatographic patterns. When mixtures of solvents with different vapour pressures are used as the mobile phase, saturation of the tank is a critical point for obtaining reproducible chromatograms. A chromatographic tank designed to give optimal saturation conditions, resulting in highly reproducible chromatograms, is described.
引用
收藏
页码:155 / 157
页数:3
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