POLLEN FERTILITY RESTORATION BY NUCLEAR GENE FR IN CMS COMMON BEAN - AN FR LINKAGE MAP AND THE MODE OF FR ACTION

被引：15

作者：

HE, S

YU, ZH

VALLEJOS, CE

MACKENZIE, SA

机构：

[1] PURDUE UNIV,DEPT AGRON,W LAFAYETTE,IN 47907

[2] UNIV FLORENCE,DEPT HORT SCI,GAINESVILLE,FL 32611

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 7-8期

关键词：

PHASEOLUS VULGARIS L; FR-DIRECTED MITOCHONDRIAL ALTERATION; FR LINKAGE MAP; SEMIDOMINANT EFFECT; MAP-BASED CLONING;

D O I：

10.1007/BF00222921

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The Fr gene in common bean, Phaseolus vulgaris L., is a unique gene for the study of plant nuclear-mitochondrial interactions because it appears to directly influence plant mitochondrial genome structure, resulting in the restoration of pollen fertility in cytoplasmic male sterile plants. This gene action is distinct from other pollen fertility restoration systems characterized to date. As a first step towards the map-based cloning of this unusual nuclear gene, we identified RAPD markers linked to Fr using bulked segregant analysis of near-isogenic lines. Using DNA gel blot hybridization, we localized the identified RAPD markers to a linkage group on the common bean RFLP map and constructed a linkage map of the Fr region using both RAPD markers and RFLP markers. Analysis of the mode of Fr action with the aid of identified Fr-linked DNA markers indicated that Fr functions in a semidominant fashion, showing dosage effect in controlling the dynamics of a heteroplasmic mitochondrial population. We also present our observations on the developmental distinctions, crucial in the accurate mapping of the Fr gene, between spontaneous cytoplasmic reversion and Fr-driven fertility restoration, two phenomena that are phenotypically indistinguishable.

引用

页码：1056 / 1062

页数：7

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