NUCLEAR ASSEMBLY IS INDEPENDENT OF LINKER HISTONES

被引：64

作者：

DASSO, M ^{[1
]}

DIMITROV, S ^{[1
]}

WOLFFE, AP ^{[1
]}

机构：

[1] NICHHD,MOLEC EMBRYOL LAB,MOLEC BIOL SECT,BETHESDA,MD 20892

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 26期

关键词：

NUCLEUS; CHROMATIN; XENOPUS;

D O I：

10.1073/pnas.91.26.12477

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free extract of Xenopus eggs that transforms condensed sperm chromatin into DNA replication-competent pronuclei. When linker histones were removed from the extract, the resultant pronuclei were indistinguishable from those formed in the complete extract. The assembly of functional nuclear membrane, nuclear lamina, and prereplication centers allowed identical DNA replication efficiencies. Thus, linker histones are not required for the assembly of morphologically normal nuclei capable of DNA replication.

引用

页码：12477 / 12481

页数：5

共 43 条

[1]

IDENTIFICATION OF NUCLEAR PREREPLICATION CENTERS POISED FOR DNA-SYNTHESIS IN XENOPUS EGG EXTRACTS - IMMUNOLOCALIZATION STUDY OF REPLICATION PROTEIN-A [J].

ADACHI, Y ;

LAEMMLI, UK .

JOURNAL OF CELL BIOLOGY, 1992, 119 (01) :1-15

[2]

INITIATION OF DNA-REPLICATION IN NUCLEI AND PURIFIED DNA BY A CELL-FREE-EXTRACT OF XENOPUS EGGS [J].

BLOW, JJ ;

LASKEY, RA .

CELL, 1986, 47 (04) :577-587

[3]

BRADBURY EM, 1992, BIOESSAYS, V14, P9

[4]

THE NUCLEOSKELETON AND THE TOPOLOGY OF REPLICATION [J].

COOK, PR .

CELL, 1991, 66 (04) :627-635

[5]

DASSO M, 1988, MOL CELL BIOL, V12, P3337

[6]

CHROMATIN TRANSITIONS DURING EARLY XENOPUS EMBRYOGENESIS - CHANGES IN HISTONE-H4 ACETYLATION AND IN LINKER HISTONE TYPE [J].

DIMITROV, S ;

ALMOUZNI, G ;

DASSO, M ;

WOLFFE, AP .

DEVELOPMENTAL BIOLOGY, 1993, 160 (01) :214-227

[7]

REMODELING SPERM CHROMATIN IN XENOPUS-LAEVIS EGG EXTRACTS - THE ROLE OF CORE HISTONE PHOSPHORYLATION AND LINKER HISTONE B4 IN CHROMATIN ASSEMBLY [J].

DIMITROV, S ;

DASSO, MC ;

WOLFFE, AP .

JOURNAL OF CELL BIOLOGY, 1994, 126 (03) :591-601

[8]

THE MATERNAL HISTONE H1 VARIANT, H1M (B4 PROTEIN), IS THE PREDOMINANT H1 HISTONE IN XENOPUS-PREGASTRULA EMBRYOS [J].

DWORKINRASTL, E ;

KANDOLF, H ;

SMITH, RC .

DEVELOPMENTAL BIOLOGY, 1994, 161 (02) :425-439

[9]

CELLULAR FACTORS REQUIRED FOR MULTIPLE STAGES OF SV40 DNA-REPLICATION INVITRO [J].

FAIRMAN, MP ;

STILLMAN, B .

EMBO JOURNAL, 1988, 7 (04) :1211-1218

[10]

FANG F, 1993, J CELL SCI, V106, P983

← 1 2 3 4 5 →