SIMULTANEOUS DETERMINATION OF ACETYLSALICYLIC-ACID AND ITS MAJOR METABOLITES IN HUMAN SERUM BY 2ND-DERIVATIVE SYNCHRONOUS FLUORESCENCE SPECTROMETRY

A method is described for the simultaneous determination of acetylsalicylic, salicylic, gentisic and salicyluric acids (ASA, SA, GA and SU, respectively) in serum, based on their native fluorescence. The ASA-SA-GA-SU-containing serum samples are extracted with chloroform-1% acetic acid solution; ASA and SA are determined in the organic phase, and GA and SU in the aqueous phase, after removal of protein with trichloroacetic acid, at pH 5.0 and 11.6, respectively. The ASA-SA and GA-SU-SA mixtures are resolved using second-derivative fluorescence spectrometry and the appropriate empirical equations involving the effect of each acid on the signal of the other. Recoveries from sera spiked with ASA (1.0-10-mu-g ml-1), SA (25-50-mu-g ml-1), GA (0.05-0.2-mu-g ml-1) and SU (1.0-5.0-mu-g ml-1) ranged from 100 to 104% (mean 101%), from 93 to 99% (mean 97%), from 94 to 104% (mean 99%) and from 94 to 107% (mean 98%), respectively.