RAT KC CDNA CLONING AND MESSENGER-RNA EXPRESSION IN LUNG MACROPHAGES AND FIBROBLASTS

被引：74

作者：

HUANG, SL

PAULAUSKIS, JD

KOBZIK, L

机构：

[1] HARVARD UNIV,SCH PUBL HLTH,DEPT ENVIRONM HLTH,BOSTON,MA 02115

[2] BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90679-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and sequenced overlapping cDNA clones for rat KC{black star}. The 0.93 kb cDNA has a single open reading frame of 288 nucleotides, and substantial sequence identity with the platelet-factor 4 family members mouse KC, hamster gro, and human gro. Using cloned cDNA as a probe, expression of KC mRNA in lavaged rat alveolar macrophages (AMs) increased after lipopolysaccharide (LPS) treatment. We also studied expression in vitro by a rat fetal lung fibroblast cell line, RFL-6. Expression of KC mRNA in RFL-6 cells increased after treatment with interleukin 1 or with conditioned medium from rat AMs treated with LPS. © 1992.

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页码：922 / 929

页数：8

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