Anti-peptide antibodies against an autoimmune epitope on human muscarinic receptor mimic functional autoantibodies against the same epitope in patients with idiopathic dilated cardiomyopathy

被引：14

作者：

Fu, MLX

机构：

来源：

EUROPEAN HEART JOURNAL | 1995年 / 16卷

关键词：

muscarinic receptor-2; rabbit antibody; human autoantibody;

D O I：

10.1093/eurheartj/16.suppl_O.89

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A synthetic peptide corresponding to the sequence 169-193 of the second extracellular loop of the the human muscarinic receptor-2 was used to mise antibodies in rabbits. Affinity-purified antibodies were able to interface with muscarinic receptor radioligand binding and to recognize the muscarinic receptor protein in rats. The antibodies could also exert the physiological effects of muscarinic agonist stimulation as demonstrated by decreases in isoproterenol-stimulated cAMP accumulation in Guinea-pig ventricles heart beating frequency in cultured meonatal cardiomyocytes, the maximal rate of rise of ventricular pressure and heart rate in rats in-vivo. These results demonstrate that the second extracellular loop of the muscarinic acetylcholine receptor-2 is an immunologically and functionally important domain and that rabbit antibodies have properties comparable to those found for autoantibodies against the same epitope in patients with idiopathic dilated cardiomyopathy.

引用

页码：89 / 91

页数：3

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