PHOTODYNAMICALLY INDUCED CYTOTOXICITY OF HYPERICIN DYE ON HUMAN FIBROBLAST CELL-LINE MRC5

被引：63

作者：

HADJUR, C ^{[1
]}

RICHARD, MJ ^{[1
]}

PARAT, MO ^{[1
]}

FAVIER, A ^{[1
]}

JARDON, P ^{[1
]}

机构：

[1] CHRU A MICHALLON,BIOCHIM LAB C,F-38043 GRENOBLE,FRANCE

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1995年 / 27卷 / 02期

关键词：

ANTICANCER AGENTS; HYPERICIN; PHOTOSENSITIZATION; PHOTODYNAMIC THERAPY; SINGLET OXYGEN; SUPEROXIDE; REACTIVE OXYGEN SPECIES; HUMAN FIBROBLAST CELL;

D O I：

10.1016/1011-1344(94)07064-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The possible application of hypericin (hyp) in the photodynamic therapy (PDT) of cancer was investigated using the human fibroblast cell line MRC5. In aerobic conditions, at pH 7.4, irradiation of MRCS cells was carried out with different doses of visible light and different doses of hyp. A low concentration of hyp (5x10(-9) M) was highly toxic to MRC5 cells, producing 15% survival for an irradiation period of 40 min. In the dark, no cytotoxicity was observed in the range 10(-9)-10(-7) M hyp. The mechanism of cell killing by hyp was also examined. Significant inhibition of MRCS killing was observed on addition of 1,4-diazabicyclo[2,2,2]octane (DABCO) or histidine, known quenchers of type II mechanisms. In addition, the photodynamic effect of hyp was enhanced by deuterium oxide. The addition of desferrioxamine, catalase or superoxide dismutase (SOD), known scavenging agents of the type I mechanism, had a significant inhibitory effect on the rate of photodynamic action of hyp. The experimental results suggest that hyp has considerable potential for use as a sensitizer in the PDT of cancer.

引用

页码：139 / 146

页数：8

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