DETERMINATION OF TRICHOTHECENES IN CEREALS BY GAS-CHROMATOGRAPHY WITH ION TRAP DETECTION

被引：34

作者：

SCHWADORF, K ^{[1
]}

MULLER, HM ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST ANIM NUTR,EMIL WOLFF STR 10,W-7000 STUTTGART 70,GERMANY

来源：

CHROMATOGRAPHIA | 1991年 / 32卷 / 3-4期

关键词：

GAS-CHROMATOGRAPHY MASS-SPECTROMETRY; ION TRAP DETECTOR; TRICHOTHECENES;

D O I：

10.1007/BF02325016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for determination of the trichothecene toxins, deoxynivalenol, 3-alpha-acetyl-deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin and diacetoxyscirpenol in cereals (wheat, barley, oats, corn) is described. Extraction was performed according to Tanaka et al. (J. Chromatogr. 328,271 (1985)) [33], derivatization by trifluoroacylation with trifluoroacetic acid anhydride. For quantitation and confirmation a capillary gas chromatograph combined with a selective mass detector (ion trap) working in CI-mode with methanol as reagent gas was used. The quantitation limit for the complete method is 1-5-mu-g/kg, depending on the chemical characteristics of each toxin and cleanness of the extracts. Recoveries from spiked cereals were 78-89%.

引用

页码：137 / 142

页数：6

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