LOCALIZATION OF PROTEINS FORMING THE OUTER SURFACE OF ISOLATED METAPHASE CHROMOSOMES

被引：7

作者：

BELYAEV, ND

BUDKER, VG

DUBROVSKAYA, VA

KIM, AA

KISELEVA, EV

SIDOROV, VN

机构：

[1] ACAD SCI UZSSR,INST NUCL PHYS,BIOL RADIOCHEM LAB,ULUGBEK 702132,UZBEKISTAN,USSR

[2] ACAD SCI USSR,INST CYTOL & GENET,NOVOSIBIRSK 630090,USSR

[3] ACAD SCI USSR,INST BIOORGAN CHEM,NOVOSIBIRSK 630090,USSR

来源：

FEBS LETTERS | 1992年 / 297卷 / 1-2期

关键词：

METAPHASE CHROMOSOME; NONHISTONE PROTEIN; SCAFFOLD;

D O I：

10.1016/0014-5793(92)80323-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.

引用

页码：43 / 45

页数：3

共 8 条

[1] MEMBRANE-MEDIATED CHANGES IN THE STRUCTURE OF CHROMOSOMES [J].