NITRIC-OXIDE SYNTHASE INHIBITION WITH N-G-MONO-METHYL-L-ARGININE REVERSIBLY DECREASES CEREBRAL BLOOD BOW IN PIGLETS

Qbjective: We tested the hypothesis that, in piglets, the intravenous administration of the reversible inhibitor of nitric oxide synthase, N-G-mono-methyl-L-arginine, decreases cerebral blood flow via a mechanism unrelated to cerebral oxygen consumption. Design: Prospective, randomized, controlled animal study. Setting: Animal laboratory at a university. Subjects: Pentobarbital-anesthetized piglets (1 to 2 wks of age; 2.6 to 4.0 kg). Interventions: Piglets were treated with either 50 mg of N-G-mono-methyl-L-arginine, 100 mg of N-G-mono-methyl-L-arginine, or an equal volume of saline by intravenous infusion over 10 mim. Measurements and Main Results: Mean arterial pressure increased after N-G-mono-methyl-L-arginine (50 mg dose: 84+/-6 to 100+/-7 mm Hg; 100 mg dose: 82+/-4 to 107+/-4 mm Hg; p<.001). Forebrain blood flow (microspheres) decreased (37+/-2 to 30+/-2 mL/min/100 g;p<.05) and cerebrovascular resistance increased (2.1+/-0.2 to 3.5+/-0.3 mm Ng/mL/min/100 g;p<.05) only after 100 mg of N-G-mono-methyl-L-arginine. Neurohypophysis blood flow decreased to 56+/-9% of the control value, while forebrain blood flow decreased only to 81+/-4% of the control value after 100 mg of NG-mono methyl-L-arginine administration. Blood flow returned to control values by 30 mins after infusion. N-G-mono-methyl-L-arginine administration had no effect on cerebral oxygen consumption at either dose. Intravenous administration of L-arginine (300 mg) immediately after the infusion of 100 mg of N-G-mono-methyl-L-arginine was associated with prompt (by 3 mins) recovery of blood flow to all brain regions that were affected by NG-mono-methyl-L-arginine. Conclusions: These data suggest that nitric oxide and/or a nitric oxide-containing substance is an important mediator of cerebrovascular tone in piglets, acting via a mechanism unrelated to altering cerebral oxygen consumption.