SER-127-TO-LEU SUBSTITUTION IN THE DNA GYRASE-B SUBUNIT OF STREPTOCOCCUS-PNEUMONIAE IS IMPLICATED IN NOVOBIOCIN RESISTANCE

被引：48

作者：

MUNOZ, R ^{[1
]}

BUSTAMANTE, M ^{[1
]}

DELACAMPA, AG ^{[1
]}

机构：

[1] INST SALUD CARLOS 3,CTR NACL MICROBIOL,UNIDAD GENET BACTERIANA,E-28220 MAJADAHONDA,SPAIN

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 14期

关键词：

D O I：

10.1128/jb.177.14.4166-4170.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the cloning of the gyrB gene from Streptococcus pneumoniae 533 that carries the nov-1 allele. The gyrB gene codes for a protein homologous to the gyrase B subunit of archaebacteria and eubacteria. The same amino acid substitution (Ser-127 to Leu) confers novobiocin resistance on four isolates of S. pneumoniae. This amino acid position is equivalent to Val-120 of Escherichia coli GyrB, a residue that lies inside the ATP-binding domain as revealed by the crystal structure of the protein.

引用

页码：4166 / 4170

页数：5

共 40 条

[1] DNA-SEQUENCE OF THE ESCHERICHIA-COLI GYRB GENE - APPLICATION OF A NEW SEQUENCING STRATEGY [J].

ADACHI, T ;

MIZUUCHI, M ;

ROBINSON, EA ;

APPELLA, E ;

ODEA, MH ;

GELLERT, M ;

MIZUUCHI, K .

NUCLEIC ACIDS RESEARCH, 1987, 15 (02) :771-784

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3] 3-DIMENSIONAL MODEL OF ESCHERICHIA-COLI GYRASE-B SUBUNIT CRYSTALLIZED IN 2-DIMENSIONS ON NOVOBIOCIN-LINKED PHOSPHOLIPID FILMS [J].

CELIA, H ;

HOERMANN, L ;

SCHULTZ, P ;

LEBEAU, L ;

MALLOUH, V ;

WIGLEY, DB ;

WANG, JC ;

MIOSKOWSKI, C ;

OUDET, P .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (02) :618-628

[4] CONSTRUCTION AND PROPERTIES OF A NEW INSERTION VECTOR, PJDC9, THAT IS PROTECTED BY TRANSCRIPTIONAL TERMINATORS AND USEFUL FOR CLONING OF DNA FROM STREPTOCOCCUS-PNEUMONIAE [J].

CHEN, JD ;

MORRISON, DA .

GENE, 1988, 64 (01) :155-164

[5]

CONTRERAS A, 1992, MOL MICROBIOL, V6, P1617

[6] AN UNUSUAL MECHANISM FOR RESISTANCE TO THE ANTIBIOTIC COUMERMYCIN-A1 [J].

DELCASTILLO, I ;

VIZAN, JL ;

RODRIGUEZSAINZ, MD ;

MORENO, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8860-8864

[7] MOLECULAR-BASIS OF TBE OPTOCHIN-SENSITIVE PHENOTYPE OF PNEUMOCOCCUS - CHARACTERIZATION OF THE GENES ENCODING THE F-0 COMPLEX OF THE STREPTOCOCCUS-PNEUMONIAE AND STREPTOCOCCUS-ORALIS H+-ATPASES [J].

FENOLL, A ;

MUNOZ, R ;

GARCIA, E ;

DELACAMPA, AG .

MOLECULAR MICROBIOLOGY, 1994, 12 (04) :587-598

[8] GENE LOCALIZATION, SIZE, AND PHYSICAL MAP OF THE CHROMOSOME OF STREPTOCOCCUS-PNEUMONIAE [J].

GASC, AM ;

KAUC, L ;

BARRAILLE, P ;

SICARD, M ;

GOODGAL, S .

JOURNAL OF BACTERIOLOGY, 1991, 173 (22) :7361-7367

[9] DNA GYRASE - ENZYME THAT INTRODUCES SUPERHELICAL TURNS INTO DNA [J].

GELLERT, M ;

MIZUUCHI, K ;

ODEA, MH ;

NASH, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (11) :3872-3876

[10] NALIDIXIC-ACID RESISTANCE - 2ND GENETIC CHARACTER INVOLVED IN DNA GYRASE ACTIVITY [J].

GELLERT, M ;

MIZUUCHI, K ;

ODEA, MH ;

ITOH, T ;

TOMIZAWA, JI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) :4772-4776

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