MOLECULAR DOSIMETRY OF SISTER CHROMATID EXCHANGE INDUCTION IN 9L CELLS TREATED WITH 6-THIOGUANINE

被引：15

作者：

BODELL, WJ

机构：

[1] Brain Tumor Research Center, Department of Neurological Surgery, School of Medicine, University of California, San Francisco, San Francisco, CA 94143

来源：

MUTAGENESIS | 1991年 / 6卷 / 03期

关键词：

D O I：

10.1093/mutage/6.3.175

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The induction of sister chromatid exchanges (SCE) in 9L cells treated with 6-thioguanine (6-TG) has been investigated. A 24 h treatment with 0.2-mu-M 6-TG induced approximately 28 SCE/metaphase. The dose-response curve was linear at doses below 0.2-mu-M and had a slope of 139 SCE/metaphase/mu-M 6-TG. At concentrations of 0.023 to 1-mu-M, incorporation of 6-TG in DNA was linear with dose. The slope of the dose-response curve was 4135-mu-mol 6-TG/mol DNA/mu-M 6-TG. Comparison of these results with those obtained in our previous studies of the monofunctional alkylating agent ethylnitrosourea and the bifunctional alkylating agent 3-(4-amino-2-methyl-5-pyrimidinyl)methyl-1-(2-chloroethyl)-1-nitrosourea suggest that to accurately estimate the effectiveness of particular DNA modifications at inducing SCE, the extent of formation of these DNA modifications must be known.

引用

页码：175 / 177

页数：3

共 18 条

[1]

BARKER M, 1973, CANCER RES, V33, P976

[2] REDUCED DNA-REPAIR IN MOUSE SATELLITE DNA AFTER TREATMENT WITH METHYLMETHANESULFONATE, AND N-METHYL-N-NITROSOUREA [J].

BODELL, WJ ;

BANERJEE, MR .

NUCLEIC ACIDS RESEARCH, 1976, 3 (07) :1689-1701

[3] POTENTIATION OF 1,3-BIS(2-CHLOROETHYL)-1-NITROSOUREA (BCNU)-INDUCED CYTO-TOXICITY IN 9L-CELLS BY PRETREATMENT WITH 6-THIOGUANINE [J].

BODELL, WJ ;

MORGAN, WF ;

RASMUSSEN, J ;

WILLIAMS, ME ;

DEEN, DF .

BIOCHEMICAL PHARMACOLOGY, 1985, 34 (04) :515-520

[4]

BODELL WJ, 1984, CANCER RES, V44, P3763

[5] COMPARISON OF SISTER-CHROMATID EXCHANGE INDUCTION CAUSED BY NITROSOUREAS THAT ALKYLATE OR ALKYLATE AND CROSSLINK DNA [J].

BODELL, WJ ;

AIDA, T ;

RASMUSSEN, J .

MUTATION RESEARCH, 1985, 149 (01) :95-100

[6]

BODELL WJ, 1985, CANCER RES, V45, P3460

[7] THE INDUCTION OF SCE AND CHROMOSOMAL-ABERRATIONS WITH RELATION TO SPECIFIC BASE METHYLATION OF DNA IN CHINESE-HAMSTER CELLS BY N-METHYL-N-NITROSOUREA AND DIMETHYL SULFATE [J].

CONNELL, JR ;

MEDCALF, ASC .

CARCINOGENESIS, 1982, 3 (04) :385-390

[8] INDUCTION OF MUTATIONS AND SISTER-CHROMATID EXCHANGES IN CHINESE-HAMSTER OVARY CELLS BY ETHYLATING AGENTS - RELATIONSHIP TO SPECIFIC DNA ADDUCTS [J].

HEFLICH, RH ;

BERANEK, DT ;

KODELL, RL ;

MORRIS, SM .

MUTATION RESEARCH, 1982, 106 (01) :147-161

[9]

KRENITSKY TA, 1969, J BIOL CHEM, V244, P1263

[10] DIFFERENTIAL CHROMATID DAMAGE INDUCED BY 6-THIOGUANINE IN CHO-CELLS [J].

MAYBAUM, J ;

MANDEL, HG .

EXPERIMENTAL CELL RESEARCH, 1981, 135 (02) :465-468

← 1 2 →